1K7K
crystal structure of RdgB- inosine triphosphate pyrophosphatase from E. coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97937, 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.180, 78.180, 80.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.090 - 1.500 |
| R-factor | 0.21261 |
| Rwork | 0.211 |
| R-free | 0.23751 |
| Structure solution method | MAD |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 40104 | |
| Completeness [%] | 99.3 | 93.8 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | PEG4k, Am. Acetate, Na-Citrate, pH 5.8, VAPOR DIFFUSION | |
| 1 | VAPOR DIFFUSION | 5.8 | PEG4k, Am. Acetate, Na-Citrate, pH 5.8, VAPOR DIFFUSION |
