1K7J
Structural Genomics, protein TF1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.94656 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.260, 56.260, 135.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.840 - 1.400 |
| Rwork | 0.195 |
| R-free | 0.20300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DTCOLLECT |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.084 | 0.293 |
| Number of reflections | 49419 | |
| <I/σ(I)> | 27.03 | 2.63 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 12.57 | 4.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 30% Ammonium sulfate, 0.1M Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
