1K79
Ets-1(331-440)+GGAA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.740, 74.580, 79.140 |
| Unit cell angles | 90.00, 116.14, 90.00 |
Refinement procedure
| Resolution | 17.100 * - 2.400 |
| R-factor | 0.228 * |
| Rwork | 0.224 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ets-1(331-440) bound to DNA from the Pax5(1-149)+Ets-1(331-440)+DNA complex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.050 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.061 | 0.278 |
| Number of reflections | 17326 | |
| <I/σ(I)> | 18.7 | 2.7 |
| Completeness [%] | 89.9 | 58.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.4M-1.6M Sodium Citrate, 100mM Hepes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | Hepes |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.5 (mM) | |
| 2 | 1 | reservoir | sodium citrate | 1.4-1.6 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH6.5 |
