1K6Q
Crystal structure of antibody Fab fragment D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8350 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.170, 77.480, 75.190 |
| Unit cell angles | 90.00, 114.75, 90.00 |
Refinement procedure
| Resolution | 19.600 - 2.400 |
| Rwork | 0.225 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | humanised Fab D3h44 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.580 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 16837 | |
| <I/σ(I)> | 11.7 | 4.4 |
| Completeness [%] | 94.4 | 84.5 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.6 | 293 | lithium sulphate, sodium acetate, pH 3.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
