1K6I
Crystal structure of Nmra, a negative transcriptional regulator (Trigonal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.9537 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.800, 76.800, 104.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.890 - 1.800 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25800 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.139 |
Number of reflections | 29825 | |
<I/σ(I)> | 19.4 | 9.2 |
Completeness [%] | 88.5 | 51.6 |
Redundancy | 10.6 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | potassium chloride, sodium dihydrogen phosphate, potassium dihydrogen phosphate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |