1K5V

Human acidic fibroblast growth factor. 141 amino acid form with amino terminal His tag with Asn106 replaced by Gly (N106G).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2001-04-11
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.600, 60.120, 101.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 2.100
R-factor	0.226 *
Rwork	0.226
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqz
RMSD bond length	0.008
RMSD bond angle	16.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.400	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.050	0.176
Total number of observations	245767 *
Number of reflections	14287 *
<I/σ(I)>	44.1	10.8
Completeness [%]	97.4 *	94.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298.15	PEG20,000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NaPi	50 (mM)
2	1	drop		0.1 (M)
3	1	drop	ammonium sulfate	10 (mM)
4	1	drop	EDTA	0.5 (mM)
5	1	drop	dithiothreitol	2 (mM)
6	1	reservoir	PEG20000	20 (%)
7	1	reservoir	MES	0.1 (M)	pH6.5