1K4L
Crystal Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase in complex with two Manganese ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.366, 88.346, 43.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.26700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1K49 but with no waters. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.490 |
| Total number of observations | 106303 * | |
| Number of reflections | 26458 | |
| <I/σ(I)> | 21.5 | 2 |
| Completeness [%] | 94.3 | 70.6 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Liao, D.-I., (2000) Acta Crystallogr, D56, 1495. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | PEG5000 MME | 24-30 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | MES-NaOH | 0.1 (M) | pH6.0-6.5 |
