1K2V
E. COLI PERIPLASMIC PROTEIN FHUD COMPLEXED WITH DESFERAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 63 |
| Unit cell lengths | 85.570, 85.570, 91.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 * |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.24200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efd |
| RMSD bond length | 0.702 |
| RMSD bond angle | 1.098 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 2.000 * | 1.970 |
| Rmerge | 0.049 | 0.205 |
| Number of reflections | 22886 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 99.1 * | 97.8 * |
| Redundancy | 99.1 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | Na/K phosphate, HEPES, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.3 (mg/ml) | |
| 2 | 1 | drop | disodium/potassium phosphate | 0.8 (M) | |
| 3 | 1 | drop | HEPES | 0.05 (M) | pH7.5 |
| 4 | 1 | reservoir | disodium/potassium phosphate | 1.6 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
