1K1B
Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.350, 50.907, 59.495 |
| Unit cell angles | 90.00, 102.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ikn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 * | 0.260 * |
| Total number of observations | 49074 * | |
| Number of reflections | 14381 | |
| <I/σ(I)> | 15.1 | 3.15 |
| Completeness [%] | 98.6 | 94.4 |
| Redundancy | 3.41 | 2.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 * | 20 * | PEG 6000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 20 (mM) | |
| 2 | 1 | drop | 350 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | protein | 3-10 (mg/ml) | |
| 5 | 1 | reservoir | PEG6000 | 5 (%) | |
| 6 | 1 | reservoir | MES | 100 (mM) |
