1K1A
Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.703, 51.218, 64.680 |
| Unit cell angles | 90.00, 102.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.860 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.22859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ikn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 * | 0.697 * |
| Total number of observations | 46088 * | |
| Number of reflections | 17021 | |
| <I/σ(I)> | 10.5 | 1.65 |
| Completeness [%] | 89.8 | 77.8 |
| Redundancy | 2.71 * | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 * | 20 * | PEG 6000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 20 (mM) | |
| 2 | 1 | drop | 350 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | protein | 3-10 (mg/ml) | |
| 5 | 1 | reservoir | PEG6000 | 5 (%) | |
| 6 | 1 | reservoir | MES | 100 (mM) |
