1K12
Fucose Binding lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 65.540, 65.540, 245.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.400 * - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.237 |
| Number of reflections | 16454 | |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 9.71 | 5.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | MPD 40-50%, 5mM Fucose 19 mM Na Cl and 10 mM Tris HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 40-50 (%(v/v)) | pH7-9 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | alpha-L-fucose | 5 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 6 | 1 | drop | 1 (mM) |
