1K0W
CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-05 |
| Detector | BRANDEIS - B1.2 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 105.900, 105.900, 274.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jdi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.325 |
| Number of reflections | 84503 | |
| <I/σ(I)> | 23 | 3.1 |
| Completeness [%] | 93.9 | 72.1 |
| Redundancy | 6.652 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 4.0M sodium formate, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
