1K0O

Crystal structure of a soluble form of CLIC1. An intracellular chloride ion channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-18
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 1 21 1
Unit cell lengths	46.408, 60.311, 89.428
Unit cell angles	90.00, 92.41, 90.00

Refinement procedure

Resolution	87.710 - 1.750
R-factor	0.243 *
Rwork	0.240
R-free	0.27200 *
Structure solution method	MIR
RMSD bond length	0.017 *
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CCP4
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.044	0.460
Total number of observations	212823 *
Number of reflections	48235 *
<I/σ(I)>	9.7	1.8
Completeness [%]	98.2 *	97 *
Redundancy	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5	4 *	PEG MME 5K, ammonium sulphate, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-20 (mg/ml)
2	1	drop	n-octyl-beta-D-glucopyranoside	0.5 (%)
3	1	reservoir	PEG5000 MME	14-18 (%)
4	1	reservoir	ammonium sulfate	0-30 (mM)
5	1	reservoir	sodium acetate	0.1 (M)	pH5.0