1JZT

Crystal structure of yeast ynu0, YNL200c

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-16
Detector	BRANDEIS - B1
Wavelength(s)	0.9788, 0.9785, 0.9400
Spacegroup name	P 21 21 21
Unit cell lengths	57.732, 68.676, 125.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.260 - 1.940
R-factor	0.2
Rwork	0.200
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.417
Number of reflections	39366
<I/σ(I)>	12.6	5.8
Completeness [%]	98.2	92.3
Redundancy	11	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	ammonium formate, PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K