1JZN

crystal structure of a galactose-specific C-type lectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.8931
Spacegroup name	C 2 2 21
Unit cell lengths	63.309, 178.486, 137.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 2.200
R-factor	0.198
Rwork	0.198
R-free	0.25000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	24.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.063 *	0.156 *
Number of reflections	38575
Completeness [%]	96.2	79.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	277	Na acetate pH 4.6, 50 mM lactose, 40 mM CaCl2, 1.5 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7.5 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.0
3	1	drop		1 (mM)
4	1	drop	lactose	100 (mM)
5	1	reservoir		1.3-1.5 (M)
6	1	reservoir	sodium acetate	1.3-1.5 (M)	pH4.6
7	1	reservoir		40 (mM)