1JYV
E. COLI (lacZ) BETA-GALACTOSIDASE (E537Q) IN COMPLEX WITH ONPG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2001-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.492, 167.455, 200.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 1.750 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.280 |
| Number of reflections | 491766 | |
| <I/σ(I)> | 9 | 2.6 |
| Completeness [%] | 97.0 * | 94 |
| Redundancy | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 288 | used macroseeding, Juers, D.H., (2000) Protein Sci., 9, 1685. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 2 | 1 | drop | Bis-Tris | 100 (mM) | pH6.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | pH6.5 |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | 100 (mM) |
