1JY5

RNase-related protein from Calystegia sepium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-16
Detector	MARRESEARCH
Wavelength(s)	0.9116
Spacegroup name	P 62
Unit cell lengths	121.687, 121.687, 66.059
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.630 - 2.050
R-factor	0.2074 *
Rwork	0.207
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dix
RMSD bond length	0.005
RMSD bond angle	1.230 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.063	0.338
Total number of observations	163993 *
Number of reflections	35416 *	1739 *
<I/σ(I)>	20.71	5.12
Completeness [%]	85.5	66.3 *
Redundancy	4.63	4.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	NaCl, 0.1M acetate buffer, MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		0.2 (M)
2	1	drop	acetate	0.1 (M)	pH4.6
3	1	drop	MPD	30 (%)
4	1	drop	protein	10 (mg/ml)