1JXQ
Structure of cleaved, CARD domain deleted Caspase-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 111 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.300, 81.800, 125.400 |
| Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.800 |
| R-factor | 0.233 * |
| Rwork | 0.233 |
| R-free | 0.27600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Caspase-3 PDB entry 1CP3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 | 0.283 |
| Total number of observations | 112744 * | |
| Number of reflections | 33394 | |
| <I/σ(I)> | 14.8 | 3.9 |
| Completeness [%] | 99.4 | 93.4 * |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 * | 298 | 0.1M Mes pH 6.5, 12% PEG 20,000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8-10 (mg/ml) | |
| 2 | 1 | 1 | Tris | 10 (mM) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | dithiothreitol | 1 (mM) | pH8.0 |
| 5 | 1 | 2 | MES | 0.1 (M) | pH6.5 |
| 6 | 1 | 2 | PEG20000 | 12 (%) |
