1JXK
Role of ethe mobile loop in the mehanism of human salivary amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.900, 75.737, 134.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.420 - 1.900 |
| R-factor | 0.17615 |
| Rwork | 0.173 |
| R-free | 0.19997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.057 |
| Total number of observations | 174537 * | |
| Number of reflections | 38401 | |
| <I/σ(I)> | 21.9 | 8.6 |
| Completeness [%] | 98.3 | 99 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | MPD, calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | reservoir | MPD | 40 (%(v/v)) |
