1JX6
CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-14 |
| Detector | BRANDEIS |
| Wavelength(s) | 1.0720 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.268, 77.467, 52.008 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23900 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MARMAD |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.035 * | 0.250 * |
| Number of reflections | 61620 | |
| <I/σ(I)> | 42 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | 298 | PEG 4000, GLYCEROL, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | LuxP | 8 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 0.1 (M) | pH8.5 |
| 3 | 1 | 1 | PEG4000 | 15 (%) | |
| 4 | 1 | 1 | glycerol | 18 (%(v/v)) |
