1JWY
CRYSTAL STRUCTURE OF THE DYNAMIN A GTPASE DOMAIN COMPLEXED WITH GDP, DETERMINED AS MYOSIN FUSION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.550, 61.950, 181.070 |
Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
Resolution | 29.400 - 2.300 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1JX2 (SAME PROTEIN WITHOUT NUCLEOTIDE) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.054 * | 0.186 * |
Number of reflections | 50492 | |
<I/σ(I)> | 15.7 | 4.1 |
Completeness [%] | 94.0 | 53.6 |
Redundancy | 3.4 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 11 (%) | |
10 | 1 | drop | 1 (mM) | ||
11 | 1 | drop | dithiothreitol | 1 (mM) | |
12 | 1 | drop | sucrose | 3 (%) | |
13 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | Tris | 50 (mM) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | glucose | 10 (%) | |
6 | 1 | reservoir | MPD | 2 (%) | |
7 | 1 | reservoir | EGTA | 1 (mM) | |
8 | 1 | reservoir | dithiothreitol | 5 (mM) | |
9 | 1 | drop | Tris | 50 (mM) |