1JWF

Crystal Structure of human GGA1 VHS domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2001-05-29
Detector	FUJI
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	55.120, 55.120, 105.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.22337
Rwork	0.222
R-free	0.26137
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.557
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.044 *
Total number of observations	39125 *
Number of reflections	9580 *
Completeness [%]	95.6	89.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	PEG 3350, potassium dihydrogen phosphate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	1 (mM)	pH8.0
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG3350	17 (%(w/v))
4	1	reservoir	potassium phosphate	0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)	pH7.5