1JVX

Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	103.610, 67.580, 57.270
Unit cell angles	90.00, 113.31, 90.00

Refinement procedure

Resolution	27.560 - 2.500
R-factor	0.232 *
Rwork	0.232
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mdq
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *	3.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.066
Total number of observations	24731 *
Number of reflections	12533
<I/σ(I)>	8.1	3.6
Completeness [%]	98.7	99
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	4 *	PEG 6000, sodium MES, maltose, sodium azide, beta-mercaptoethanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5-10 (mg/ml)
2	1	drop	PEG6000	9-15 (%(w/v))
3	1	drop	MES-Na	10 (mM)
4	1	drop	maltose	1 (mM)
5	1	drop	sodium azide	0.02 (%)
6	1	reservoir	PEG6000	19-22 (%)