1JVW
TRYPANOSOMA CRUZI MACROPHAGE INFECTIVITY POTENTIATOR (TCMIP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.885 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.034, 34.587, 50.412 |
Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
Resolution | 19.000 - 1.700 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.572 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.200 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.081 * | 0.097 * |
Total number of observations | 60547 * | |
Number of reflections | 17009 | |
Completeness [%] | 95.9 | 89.8 |
Redundancy | 3.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.5 | 4 * | PMSF, Tris/HCl, pH 7.5, SMALL TUBES, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Tris-HCl | 5 (mM) | pH7.5 |
2 | 1 | 1 | PMSF | 1 (mM) |