1JVN
CRYSTAL STRUCTURE OF IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE: A TUNNEL THROUGH A (BETA/ALPHA)8 BARREL JOINS TWO ACTIVE SITES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.370, 112.173, 116.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.210 - 2.100 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.067 | 0.390 |
| Number of reflections | 73741 | |
| <I/σ(I)> | 7.3 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.3 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | 28 % PEG MME 5000, 0.2 M Ammonium sulfate, 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | 28 % PEG MME 5000, 0.2 M Ammonium sulfate, 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 22-28 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.5-7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
