1JVG
CRYSTAL STRUCTURE OF HUMAN AGX2 COMPLEXED WITH UDPGALNAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9464 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.581, 71.785, 93.561 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| Rwork | 0.201 |
| R-free | 0.23900 * |
| Structure solution method | rigid body |
| Starting model (for MR) | 1jvd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 * | 0.258 * |
| Number of reflections | 50606 | |
| <I/σ(I)> | 8.1931 | 1.8 |
| Completeness [%] | 99.0 | 98.48 |
| Redundancy | 3.42 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 20 * | PEG600, IMIDAZOLE/MALATE, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.000001 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | PEG600 | 34 (%) | |
| 6 | 1 | reservoir | imidazole/malate | 0.2 (M) | pH5.7 |
