1JVG

CRYSTAL STRUCTURE OF HUMAN AGX2 COMPLEXED WITH UDPGALNAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9464
Spacegroup name	P 1 21 1
Unit cell lengths	85.581, 71.785, 93.561
Unit cell angles	90.00, 94.01, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
Rwork	0.201
R-free	0.23900 *
Structure solution method	rigid body
Starting model (for MR)	1jvd
RMSD bond length	0.012
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.062 *	0.258 *
Number of reflections	50606
<I/σ(I)>	8.1931	1.8
Completeness [%]	99.0	98.48
Redundancy	3.42	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	PEG600, IMIDAZOLE/MALATE, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.000001 (mM)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		20 (mM)
4	1	drop		5 (mM)
5	1	reservoir	PEG600	34 (%)
6	1	reservoir	imidazole/malate	0.2 (M)	pH5.7