1JV7
BACTERIORHODOPSIN O-LIKE INTERMEDIATE STATE OF THE D85S MUTANT AT 2.25 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-10-16 |
Detector | ADSC QUANTUM |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.800, 121.300, 85.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.250 |
R-factor | 0.213 * |
Rwork | 0.213 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1brx |
RMSD bond length | 0.008 |
RMSD bond angle | 1.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 * | 2.280 |
High resolution limit [Å] | 2.200 | 2.250 |
Rmerge | 0.056 | 0.553 |
Total number of observations | 164767 * | |
Number of reflections | 11949 | |
<I/σ(I)> | 9.6 | 2.2 |
Completeness [%] | 91.5 | 94.7 |
Redundancy | 13 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | cubic lipid phase | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
1 | cubic lipid phase | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
2 | 1 | 1 | protein | 3.5 (mg/ml) | |
3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |