1JV3
CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGALNAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.735, 70.769, 95.384 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.200 |
| Rwork | 0.190 |
| R-free | 0.23300 * |
| Structure solution method | rigid body |
| Starting model (for MR) | pdb code: 1JV1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 * | 0.373 * |
| Number of reflections | 64858 | |
| <I/σ(I)> | 7.4823 | 1.84 |
| Completeness [%] | 97.6 | 98.2 * |
| Redundancy | 2.62 | 2.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 20 * | peg600, Imidazole/malate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.000001 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | PEG600 | 34 (%) | |
| 6 | 1 | reservoir | imidazole/malate | 0.2 (M) | pH5.7 |
