1JUX

1.6A Resolution Crystal Structures of the DNA Octamers d(IUATATAC) and d(ITITACAC):Binding of Two Distamycin Drugs Side-by-Side

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-06-20
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	33.260, 25.050, 28.300
Unit cell angles	90.00, 124.23, 90.00

Refinement procedure

Resolution	10.000 - 1.600
Rwork	0.149
R-free	0.20700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.300 *
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.600	1.600 *
Rmerge	0.046 *	0.162 *
Number of reflections	2737 *	381 *
Completeness [%]	92.0 *	88.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	1 (mM)	single-stranded concentration
2	1	drop	sodium cacodylate	30 (mM)	pH7.0
3	1	drop	cobalt hexammine chloride	0.1 (mM)
4	1	drop	distamycin hydrochloride	1 (mM)
5	1	reservoir	MPD	60 (%)