1JTZ
CRYSTAL STRUCTURE OF TRANCE/RANKL CYTOKINE.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-04 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.300, 82.000, 99.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CHIMERA OF PDB ENTRIES 1TNR (CHAIN A) AND 1D4V (CHAIN B) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.449 * | |
Total number of observations | 191355 * | |
Number of reflections | 16705 | |
<I/σ(I)> | 32.3 | 4.7 |
Completeness [%] | 98.2 | 100 |
Redundancy | 11.5 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 11-14% PEG 4000, 16 mM calcium chloride, 80 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | Tris-HCl | 20 (mM) | |
4 | 1 | reservoir | HEPES | 80 (mM) | |
5 | 1 | reservoir | 16 (mM) | ||
6 | 1 | reservoir | PEG4000 | 11-14 (%) |