1JTH

Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9292, 0.9795, 0.9797, 1.005
Spacegroup name	C 1 2 1
Unit cell lengths	59.400, 65.780, 80.100
Unit cell angles	90.00, 99.50, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.265 *
Rwork	0.266
R-free	0.28400 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	2.000	2.000
Rmerge	0.039	0.327
Number of reflections	18147
<I/σ(I)>	24	3.6
Completeness [%]	96.8 *	98.8
Redundancy	2.4 *	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8 *	21 *	ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		150 (mM)
3	1	drop	beta-mercaptoethanol	5 (mM)
4	1	drop	Tris	25 (mM)	pH8.0
5	1	reservoir	ammonium sulfate	1.6 (M)
6	1	reservoir	MES	100 (mM)	pH6.5
7	1	reservoir	beta-mercaptoethanol	10 (mM)