1JTH
Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9292, 0.9795, 0.9797, 1.005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.400, 65.780, 80.100 |
| Unit cell angles | 90.00, 99.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.265 * |
| Rwork | 0.266 |
| R-free | 0.28400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.327 |
| Number of reflections | 18147 | |
| <I/σ(I)> | 24 | 3.6 |
| Completeness [%] | 96.8 * | 98.8 |
| Redundancy | 2.4 * | 2.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 * | 21 * | ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 4 | 1 | drop | Tris | 25 (mM) | pH8.0 |
| 5 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 7 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) |
