1JSU

P27(KIP1)/CYCLIN A/CDK2 COMPLEX

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-02-20
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	73.800, 78.300, 137.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.192 *
Rwork	0.192
R-free	0.26000
RMSD bond length	0.012
RMSD bond angle	1.450
Data reduction software	HKL
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.056
Total number of observations	162339 *
Number of reflections	34683 *
Completeness [%]	96.1
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	HEPES	40 (mM)
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	35 (%sat)
6	1	reservoir	HEPES	40 (mM)
7	1	reservoir	dithiothreitol	5 (mM)