1JSG

CRYSTAL STRUCTURE OF P14TCL1, AN ONCOGENE PRODUCT INVOLVED IN T-CELL PROLYMPHOCYTIC LEUKEMIA, REVEALS A NOVEL B-BARREL TOPOLOGY

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	38.897, 82.839, 117.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.192
Rwork	0.192
R-free	0.25900
RMSD bond length	0.006
RMSD bond angle	31.000 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.099 *	0.288 *
Total number of observations	60407 *
Number of reflections	6870 *
Completeness [%]	99.4 *	99.9 *
Redundancy	8.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.2 (mM)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop	sodium acetate	25 (mM)	can be replaced by Succinate