1JRC
The N67A mutant of Lactococcus lactis dihydroorotate dehydrogenase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84690 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.800, 108.090, 65.800 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.177 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lactococcus lactis DHODA PDB ID 2DOR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.059 | 0.207 |
| Number of reflections | 62505 | |
| <I/σ(I)> | 22.8 | 5.3 |
| Completeness [%] | 98.8 | 87.2 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 6K, Na-acetate, TRIS-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
