1JRA
Crystal Structure of Erv2p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.630, 66.810, 60.380 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JR8 prior to refinement |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.461 |
| Number of reflections | 28807 | |
| <I/σ(I)> | 15.1 | 4.5 |
| Completeness [%] | 99.8 * | 98.3 |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 8000, sodium cacodylate, magnesium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylic acid | 0.1 (M) | pH6.2 |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 32 (%(w/v)) |
