1JQU

Are Carboxy Terminii of Helices Coded by the Local Sequence or by Tertiary Structure Contacts

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-11-14
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	74.300, 77.200, 138.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.600
R-factor	0.235 *
Rwork	0.229
R-free	0.31500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	T4 Lysozyme 2LZM
RMSD bond length	0.022 *
RMSD bond angle	2.200 *
Phasing software	CNS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.057 *
Number of reflections	22095
<I/σ(I)>	6.9	1.2
Completeness [%]	78.0 *	71.5
Redundancy	3.42	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	277	30% PEG4000, PIPES buffer ph7.0, 0.2M LiSO4, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	HEPES	20 (mM)	pH7.0
3	1	reservoir	PEG4000	30 (%)
4	1	reservoir	PIPES	0.1 (M)	pH7.0
5	1	reservoir		0.2 (M)