1JQP

dipeptidyl peptidase I (cathepsin C), a tetrameric cysteine protease of the papain family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-20
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	166.240, 166.240, 80.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.245
R-free	0.27400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.322
Number of reflections	26061
Completeness [%]	99.2
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	298	bis-tris, NaCl, dithiothreitol, EDTA, bis-tris propane, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Bis-Tris	20 (mM)
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	EDTA	2 (mM)	pH7.0
6	1	reservoir	Bis-Tris propane	0.1 (M)	pH7.5
7	1	reservoir	ammonium sulfate	1.4 (M)