1JQ0
Mutation that destabilize the gp41 core: determinants for stabilizing the SIV/CPmac envelope glycoprotein complex. Mutant structure.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-05-30 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 1.1000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 48.877, 48.877, 150.987 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 1.700 |
R-factor | 0.209 * |
Rwork | 0.209 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qbz |
RMSD bond length | 0.006 |
RMSD bond angle | 13.600 * |
Data reduction software | MADNESS |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.249 |
Total number of observations | 137718 * | |
Number of reflections | 7973 | |
<I/σ(I)> | 16.6 | 9 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 9.6 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | sodium formate | 3.8 (M) |