1JPX
Mutation that destabilize the gp41 core: determinants for stabilizing the SIV/CPmac envelope glycoprotein complex. Wild type.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-04-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9480 |
Spacegroup name | P 63 |
Unit cell lengths | 54.993, 54.993, 131.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 2.300 |
R-factor | 0.237 * |
Rwork | 0.237 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qbz |
RMSD bond length | 0.007 |
RMSD bond angle | 17.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 | 0.209 |
Total number of observations | 129420 * | |
Number of reflections | 9729 | |
<I/σ(I)> | 11.7 | 8.1 |
Completeness [%] | 97.2 | 94 |
Redundancy | 6.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium chloride, CTAB, barium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | 0.6 (M) | ||
3 | 1 | reservoir | 0.04 (M) | ||
4 | 1 | reservoir | hexadecyltrimethylammonium bromide | 0.01 (M) |