1JPQ
Crystal Structure of the Oxytricha Telomeric DNA at 1.6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 27.543, 27.543, 145.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.000 - 1.600 |
| R-factor | 0.229 * |
| Rwork | 0.225 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.094 | 0.220 * |
| Number of reflections | 8862 | |
| <I/σ(I)> | 18.6 | 6.38 |
| Completeness [%] | 96.8 | 98.4 |
| Redundancy | 1.12 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | potassium chloride, magnesium chloride, spermine, MPD, potassium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 12K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | potassium cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10.0 (mM) | ||
| 2 | 1 | drop | 40.0 (mM) | ||
| 3 | 1 | drop | 3.3 (mM) | ||
| 4 | 1 | drop | DNA | 1.0 (mM) | |
| 5 | 1 | drop | MPD | 5.0 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 25 (%(v/v)) |
