1JPI

Phe232Leu mutant of human UROD, human uroporphyrinogen III decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-01-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	103.320, 103.320, 72.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.2 *
Rwork	0.200
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uro
RMSD bond length	0.140
RMSD bond angle	2.140
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.078	0.400 *
Total number of observations	262230 *
Number of reflections	20018 *
Completeness [%]	99.0	99.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	21 *	Phillips, J.D., (1997) Protein Sci., 6, 1343. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	Tris	50 (mM)	pH7.5
3	1	drop	glycerol	10 (%)
4	1	drop	beta-mercaptoethanol	1 (mM)
5	1	reservoir	MPD	16 (%)
6	1	reservoir	MES	0.1	pH6.0