1JOW
Crystal structure of a complex of human CDK6 and a viral cyclin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.140, 70.140, 448.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 3.100 |
| R-factor | 0.266 * |
| Rwork | 0.266 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.190 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.058 * | 0.290 * |
| Total number of observations | 92567 * | |
| Number of reflections | 12678 * | |
| <I/σ(I)> | 29 | 4 |
| Completeness [%] | 97.9 * | 81.8 * |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Schulze-Gahmen, U., (2001) Acta Crystallogr, D57, 1287. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | reservoir | calcium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG3350 | 10 (%) | |
| 8 | 1 | reservoir | 0.15 (M) |
