1JOM

THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX BETWEEN FOLINIC ACID (LEUCOVORIN) AND E. COLI DIHYDROFOLATE REDUCTASE

Experimental procedure

Spacegroup name	P 65
Unit cell lengths	96.862, 96.862, 35.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.142 *
RMSD bond length	0.019
RMSD bond angle	2.996
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.068 *
Number of reflections	15022 *
Completeness [%]	99.7 *
Redundancy	7.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	L-histidine	0.5 (M)	0.0625 ml
2	1	drop	calcium acetate	0.5 (M)	0.0625 ml
3	1	drop	concentrated folnic acid-DHFR complex		0.05 ml
4	1	reservoir	ethanol	18-25 (%)
5	1	reservoir	Tris-HCl	1.0 (M)