1JOC
EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-20 |
| Detector | BRANDEIS - B1 |
| Wavelength(s) | 1.28322, 1.28356, 1.23985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.528, 85.096, 88.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.221 * |
| Rwork | 0.221 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.1) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | 11% PEG 4000, 50 mM HEPES, 60 mM ammonium acetate, 10% glycerol, and 1.5 mM Ins(1,3)P2, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 11 (%) | |
| 3 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
| 4 | 1 | reservoir | ammonium acetate | 60 (mM) | |
| 5 | 1 | reservoir | Ins(1,3)P2 | 1.5 (mM) |
