1JO8

Structural analysis of the yeast actin binding protein Abp1 SH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-09-15
Detector	MARRESEARCH
Wavelength(s)	0.842
Spacegroup name	P 21 21 21
Unit cell lengths	24.080, 38.155, 59.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.510 - 1.300
R-factor	0.14512
Rwork	0.144
R-free	0.17785
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cka
RMSD bond length	0.011
RMSD bond angle	1.714
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.056	0.266
Total number of observations	56424 *
Number of reflections	13742 *
<I/σ(I)>	20.3	4
Completeness [%]	98.0	88.6
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	Ammonium sulfate, bis-tris-propane, pH 8.0, VAPOR DIFFUSION, SITTING DROP at 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	3.2 (M)
2	1	reservoir	Bis-Tris propane	100 (mM)	pH8.0
3	1	drop	Abp1-SH3	12 (mg/ml)