1JO2
Crystal Structure of the B-DNA Hexamer (CgATCG).Daunomycin Complex Containing a Ribose at the Intercalation Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-24 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 28.050, 28.050, 53.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
Rwork | 0.198 |
R-free | 0.23300 |
RMSD bond length | 0.014 |
RMSD bond angle | 17.500 * |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 2767 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 293 | magnesium chloride, cacodylate, MPD, pH 7.00, EVAPORATION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MgCl2 | ||
2 | 1 | 1 | cacodylate | ||
3 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 2 (mM) | single strand concentration |
2 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
3 | 1 | drop | 24 (mM) | ||
4 | 1 | drop | daunomycin | 2 (mM) | |
5 | 1 | drop | MPD | 2 (%(v/v)) | |
6 | 1 | reservoir | MPD | 60 (%) |