1JNZ

Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	70
Detector technology	CCD
Collection date	1999-11-29
Detector	MARRESEARCH
Wavelength(s)	1.06
Spacegroup name	P 21 21 21
Unit cell lengths	72.600, 113.500, 193.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.190 - 2.500
R-factor	0.161
Rwork	0.161
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jnr
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.530
High resolution limit [Å]	2.500	2.500
Number of reflections	53527
Completeness [%]	79.2	71.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	PEG 4000, sodium acetate, sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K