1JNY
Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.113, 113.718, 80.320 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.224 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.172 |
| Total number of observations | 762914 * | |
| Number of reflections | 101657 | |
| <I/σ(I)> | 38.7 | 8.7 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 5.6 | 277 | Zagari, A., (1994) J. Mol. Biol., 242, 175. * |
