1JNU

Photoexcited structure of the plant photoreceptor domain, phy3 LOV2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	296
Detector technology	CCD
Collection date	2001-06-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	44.843, 54.771, 70.963
Unit cell angles	93.14, 93.79, 90.40

Refinement procedure

Resolution	30.000 * - 2.600
R-factor	0.238 *
Rwork	0.236
R-free	0.25900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	phy3 LOV2 dark-state structure
RMSD bond length	0.010
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.044	0.190
Number of reflections	17700
<I/σ(I)>	12.3	3.2
Completeness [%]	87.5	79
Redundancy	1.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	25 *	PEG 5000 MME, Tris, Glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG5000 MME	22 (%)
2	1	1	glycerol	12 (%)
3	1	1	Tris	50 (mM)	pH8.0
4	1	2	protein	15 (mg/ml)