1JNE

Crystal structure of imaginal disc growth factor-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-25
Detector	MARRESEARCH
Wavelength(s)	0.927
Spacegroup name	P 21 21 21
Unit cell lengths	49.520, 72.170, 105.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.195
Rwork	0.195
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctn
RMSD bond length	0.006
RMSD bond angle	1.540
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.043 *	0.274 *
Total number of observations	303904 *
Number of reflections	40966
<I/σ(I)>		6.05
Completeness [%]	98.2	80.8
Redundancy	1	2.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7	25 *	PEG 4000, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	PEG4000	25 (%)
2	1	2	Tris	0.1 (M)	pH7.0
3	1	1	protein	15 (mg/ml)